I have been working in the same lab for over 3 years. Many of the experiments that I do follow roughly the same protocol, especially in preparing the sample. With few exceptions, I use modified buffered salt solution (MBSS) to suspend the cells.
But today, without noticing, I grabbed the bottle labeled, "MBSS sans Ca2+/Mg2+". As John Harmon will readily tell you, "sans" means without. I don't know if that is Greek or Latin. And no one in the lab speaks either Greek or Latin, so I don't know why we don't label it as "w/o." That's faster to write than "sans." I guess that's just what we scientists do--use the fancy way to say something simple.
Anyway, basically I ruined the 3 experiments I did because there needed to be calcium in the medium for the whole thing to work. Dang it.
The thing that's most frustrating about it is that I should have realized what the problem was after the first experiment. Someone else had to (very charitably) point out the problem to me. So I felt like a robot today who just goes through the motions of doing research but isn't capable of generating real solutions to problems or really
thinking about what I'm doing.
Am I ready to convince my thesis committee that they should let me graduate? Or do I really have no idea what I'm doing? Either way, I'll be glad when April comes...